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Measuring total and germinable spore populations

Show simple item record Noell, A.C. Yung, P.T. Yang, W. Lee, C. Ponce, A. 2013-10-25T20:06:24Z 2013-10-25T20:06:24Z 2011-10-03
dc.identifier.citation EPSC-DPS Joint Meeting 2011, Nantes, France, October 2 - 07, 2011 en_US
dc.identifier.clearanceno 11-2309
dc.description.abstract It has been shown that bacterial endospores can be enumerated using a microscopy based assay that images the luminescent halos from terbium ions bound to dipicolinic acid, a spore specific chemical marker released upon spore germination. Further development of the instrument has simplified it towards automation while at the same time improving image quality. Enumeration of total spore populations has also been developed allowing measurement of the percentage of viable spores in any population by comparing the germinable/culturable spores to the total. Percentage viability will allow a more quantitative comparison of the ability of spores to survive across a wide range of extreme environments. en_US
dc.description.sponsorship NASA/JPL en_US
dc.language.iso en_US en_US
dc.publisher Pasadena, CA : Jet Propulsion Laboratory, National Aeronautics and Space Administration, 2011. en_US
dc.subject microbial life en_US
dc.subject spore enumeration en_US
dc.subject environmental sample results en_US
dc.title Measuring total and germinable spore populations en_US
dc.type Preprint en_US

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