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Microbial assessment of cabin air quality on commercial airliners

Show simple item record La Duc, Myron T. Stuecker, Tara Bearman, Gregory Venkateswaran, Kasthuri 2006-06-13T17:29:19Z 2006-06-13T17:29:19Z 2005-07-11
dc.identifier.citation International Conference on Environmental Systems, Rome, Italy, July 11, 2005 en
dc.identifier.clearanceno 05-0686
dc.description.abstract The microbial burdens of 69 cabin air samples collected from commercial airliners were assessed via conventional culture-dependent, and molecular-based microbial enumeration assays. Cabin air samples from each of four separate flights aboard two different carriers were collected via air-impingement. Microbial enumeration techniques targeting DNA, ATP, and endotoxin were employed to estimate total microbial burden. The total viable microbial population ranged from 0 to 3.6 x10 4 cells per 100 liters of air, as assessed by the ATP-assay. When these same samples were plated on R2A minimal medium, anywhere from 2% to 80% of these viable populations were cultivable. Five of the 29 samples examined exhibited higher cultivable counts than ATP derived viable counts, perhaps a consequence of the dormant nature (and thus lower concentration of intracellular ATP) of cells inhabiting these air cabin samples. Ribosomal RNA gene sequence analysis showed these samples to consist of a moderately diverse group of bacteria, including human pathogens. Enumeration of ribosomal genes via quantitative-PCR indicated that population densities ranged from 5 x 10 1 ’ to IO 7 cells per 100 liters of air. Each of the aforementioned strategies for assessing overall microbial burden has its strengths and weaknesses; this publication serves as a testament to the power of their use in concert. en
dc.description.sponsorship NASA/JPL en
dc.format.extent 2844935 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher Pasadena, CA : Jet Propulsion Laboratory, National Aeronautics and Space Administration, 2005. en
dc.subject microbe en
dc.subject aviation en
dc.subject cabin air en
dc.subject microbial diversity en
dc.title Microbial assessment of cabin air quality on commercial airliners en
dc.type Preprint en

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